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Detection of TGF-β1, HGF, IGF-1 and IGF-1R in Cleft Affected Mucosa of the Lip
Abstract
Background:
Orofacial clefts are one of the most common birth defects with multifactorial and only partly understood morphopathogenesis.
Objective:
The aim of this study was to evaluate the presence of TGF-β1, HGF, IGF-1 and IGF-1R in cleft affected mucosa of the lip.
Methods:
Lip mucosa tissue samples were obtained during surgical cleft correction from seven 2 to 6 months old children. Prepared tissue sections were stained by immunohistochemistry for TGF-β1, HGF, IGF-1 and IGF-1R. The intensity of staining was graded semiquantitatively.
Results:
We found numerous TGF-β1 and HGF-containing epithelial and connective tissue cells, moderate number of IGF-1 immunoreactive cells and even less pronounced presence of IGF-1R-positive cells.
Conclusion:
TGF-β1 and HGF are present in defective epithelia and soft tissue in cleft affected lip. Expressions of IGF-1 and IGF-1R show significant differences, and both factors play a role in the morphopathogenesis of clefts.
