Detection of TGF-β1, HGF, IGF-1 and IGF-1R in Cleft Affected Mucosa of the Lip
Elga Sidhom*, Mara Pilmane
Identifiers and Pagination:Year: 2017
First Page: 46
Last Page: 52
Publisher Id: TODJ-11-46
Article History:Received Date: 30/06/2017
Revision Received Date: 16/08/2017
Acceptance Date: 12/09/2017
Electronic publication date: 30/09/2017
Collection year: 2017
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Orofacial clefts are one of the most common birth defects with multifactorial and only partly understood morphopathogenesis.
The aim of this study was to evaluate the presence of TGF-β1, HGF, IGF-1 and IGF-1R in cleft affected mucosa of the lip.
Lip mucosa tissue samples were obtained during surgical cleft correction from seven 2 to 6 months old children. Prepared tissue sections were stained by immunohistochemistry for TGF-β1, HGF, IGF-1 and IGF-1R. The intensity of staining was graded semiquantitatively.
We found numerous TGF-β1 and HGF-containing epithelial and connective tissue cells, moderate number of IGF-1 immunoreactive cells and even less pronounced presence of IGF-1R-positive cells.
TGF-β1 and HGF are present in defective epithelia and soft tissue in cleft affected lip. Expressions of IGF-1 and IGF-1R show significant differences, and both factors play a role in the morphopathogenesis of clefts.