RESEARCH ARTICLE
Detection of TGF-β1, HGF, IGF-1 and IGF-1R in Cleft Affected Mucosa of the Lip
Elga Sidhom*, Mara Pilmane
Article Information
Identifiers and Pagination:
Year: 2017Volume: 11
First Page: 46
Last Page: 52
Publisher ID: TODJ-11-46
DOI: 10.2174/1874372201711010046
Article History:
Received Date: 30/06/2017Revision Received Date: 16/08/2017
Acceptance Date: 12/09/2017
Electronic publication date: 30/09/2017
Collection year: 2017
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
Orofacial clefts are one of the most common birth defects with multifactorial and only partly understood morphopathogenesis.
Objective:
The aim of this study was to evaluate the presence of TGF-β1, HGF, IGF-1 and IGF-1R in cleft affected mucosa of the lip.
Methods:
Lip mucosa tissue samples were obtained during surgical cleft correction from seven 2 to 6 months old children. Prepared tissue sections were stained by immunohistochemistry for TGF-β1, HGF, IGF-1 and IGF-1R. The intensity of staining was graded semiquantitatively.
Results:
We found numerous TGF-β1 and HGF-containing epithelial and connective tissue cells, moderate number of IGF-1 immunoreactive cells and even less pronounced presence of IGF-1R-positive cells.
Conclusion:
TGF-β1 and HGF are present in defective epithelia and soft tissue in cleft affected lip. Expressions of IGF-1 and IGF-1R show significant differences, and both factors play a role in the morphopathogenesis of clefts.