RESEARCH ARTICLE
Infrared Thermography in Serotonin-Induced Itch Model in Rats
Yousef Jasemian*, 1, Parisa Gazerani2, Frederik Dagnaes-Hansen1
Article Information
Identifiers and Pagination:
Year: 2012Volume: 6
First Page: 1
Last Page: 7
Publisher ID: TODJ-6-1
DOI: 10.2174/1874372201206010001
Article History:
Received Date: 02/02/2011Revision Received Date: 31/05/2011
Acceptance Date: 06/06/2011
Electronic publication date: 26/1/2012
Collection year: 2012
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The study validated the application of infrared thermography in a serotonin-induced itch model in rats since the only available method in animal models of itch is the count of scratching bouts. Twenty four adult Sprague-Dawley male rats were used in 3 experiments: 1) local vasomotor response induced by intradermal serotonin (10 μl) was evaluated against isotonic saline and Methysergide (10μl); 2) dose-temperature relation of intradermal serotonin with different concentrations (1%, 2%, 4%) at the site of injection was tested; 3) the local vasomotor responses in anaesthetized rats with no scratching reflex was investigated. Serotonin elicited significant scratching and lowered the local temperature at the site of injection. A negative dose-temperature relationship of serotonin was found by thermography. Vasoregulation at the site of serotonin injection took place in the absence of scratching reflexes. Thermography is a reliable, non-invasive, and objective method for assessment in serotonin-induced itch model in rat.