RESEARCH ARTICLE


Possible Role of Phosphatidylglycerol-Activated Protein Kinase C-βII in Keratinocyte Differentiation



Lakiea J. Bailey2, Vivek Choudhary1, 2, Wendy B. Bollag*, 1, 2
1 Charlie Norwood VA Medical Center, One Freedom Way, Augusta, GA 30904, USA
2 Department of Physiology, 1120 15th Street, Medical College of Georgia at Augusta University (formerly Georgia Regents University), Augusta, GA 30912, USA


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 2183
Abstract HTML Views: 1539
PDF Downloads: 935
ePub Downloads: 840
Total Views/Downloads: 5497
Unique Statistics:

Full-Text HTML Views: 680
Abstract HTML Views: 585
PDF Downloads: 381
ePub Downloads: 265
Total Views/Downloads: 1911



© 2017 Bailey et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Physiology, 1120 15th Street, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA, Tel: (706) 721-0698, Fax: (706) 721-7299, E-mail: wbollag@augusta.edu


Abstract

Background:

The epidermis is a continuously regenerating tissue maintained by a balance between proliferation and differentiation, with imbalances resulting in skin disease. We have previously found that in mouse keratinocytes, the lipid-metabolizing enzyme phospholipase D2 (PLD2) is associated with the aquaglyceroporin, aquaporin 3 (AQP3), an efficient transporter of glycerol. Our results also show that the functional interaction of AQP3 and PLD2 results in increased levels of phosphatidylglycerol (PG) in response to an elevated extracellular calcium level, which triggers keratinocyte differentiation. Indeed, we showed that directly applying PG can promote keratinocyte differentiation.

Objective:

We hypothesized that the differentiative effects of this PLD2/AQP3/PG signaling cascade, in which AQP3 mediates the transport of glycerol into keratinocytes followed by its PLD2-catalyzed conversion to PG, are mediated by protein kinase CβII (PKCβII), which contains a PG-binding domain in its carboxy-terminus. Method: To test this hypothesis we used quantitative RT-PCR, western blotting and immunocytochemistry.

Results:

We first verified the presence of PKCβII mRNA and protein in mouse keratinocytes. Next, we found that autophosphorylated (activated) PKCβII was redistributed upon treatment of keratinocytes with PG. In the unstimulated state phosphoPKCβII was found in the cytosol and perinuclear area; treatment with PG resulted in enhanced phosphoPKCβII localization in the perinuclear area. PG also induced translocation of phosphoPKCβII to the plasma membrane. In addition, we observed that overexpression of PKCβII enhanced calcium- and PG-induced keratinocyte differentiation without affecting calcium-inhibited keratinocyte proliferation.

Conclusion:

These results suggest that the PG produced by the PLD2/AQP3 signaling module may function by activating PKCβII.

Keywords: Aquaporin-3 (AQP3), Epidermis, Keratin-10, Phospholipase D2 (PLD2), Skin, Kinase.