Molecular Cryo-Electron Tomography of Skin

Lars Norlen*
Department of Cellular and Molecular Biology, Karolinska Institute, Stockholm, Sweden

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© 2010 Lars Norlen

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Cellular and Molecular Biology (CMB), Karolinska Institute, Stockholm, Sweden; Tel: 00 46 (0)8 524 87658; E-mail:


The three-dimensional structure of skin can be studied at near-native conditions at the molecular level with a new technique called molecular electron tomography of vitreous tissue sections (tissue TOVIS). Its usage is, however, still largely limited by a difficult sample handling procedure. Here we discuss some measures for the efficient application of tissue TOVIS to dermatological research.

Keywords: Skin barrier, cryo-electron microscopy of vitreous sections, CEMOVIS, cryo-electron tomography of vitreous sections, TOVIS.